Fig. S3
Def6 and Wnt11 do not act in the same linear pathway. (A) Tail-bud stage (10 hpf); GFP/def6 RNA, although detectable (green), failed to rescue the CE movement defects caused by MO-mediated knockdown of wnt11. (B) Embryos (3 dpf) were scored morphologically into three categories: normal (blue box), mild (yellow box) characterised by mild cyclopia and moderate/severe (red box) characterised by complete cyclopia and no forebrain structures anterior to the eyes. (C) The phenotypes of the embryos from three independent experiments were scored and the percentages of normal/WT (blue bar), mild (yellow bar), and moderate/severe (red bar) are indicated. Injections with GFP/def6 RNA and wnt11 MO alone were performed in parallel on the same clutch of embryos.