|
Fig. 2
Def6 morphants display defects associated with abnormal gastrulation movements.
(A–C) Zebrafish embryos uninjected (wt), or injected with 2.5 or 5 ng def6 splice MO, respectively, are shown at the 1-somite stage. (D) The angle between the anterior- and posterior- most end was measured at the 1-somite stage and the average angle is depicted in degrees. Two-tailed Student′s t-tests showed a significant (p<0.001; three asterisks) increase in the angle after injection of 2.5 ng def6 MO versus wt and an additional significant (p<0.001; three asterisks) increase in 5 ng def6 MO-injected embryos when compared to wt. (E) Def6 MO-injected embryos show a reduction in overall length at 3 dpf (wt at the top; increasing severity of phenotype in def6 MO-injected embryos towards the bottom, defined as mild, moderate and severe, respectively). (F) The length of 20 morphants injected with 2.5 ng def6 MO was measured and normalised to the length of the wt control embryos. Two-tailed Student′s t-tests showed a significant (p<0.001; three asterisks) decrease in length after injection of 2.5 ng def6 MO versus wt embryos. (G) The phenotypes of the embryos were scored at 3 dpf and the percentages of normal/mild (blue bar), moderate (green bar) and severe (red bar) morphology are shown.