|
Fig. 3
Expression pattern of dkk3a(dkk3l) during embryonic development Expression of dkk3a(dkk3l) was analyzed by whole mount in situ hybridization in embryos ranging from 6 to 72hpf. At 6hpf expression was found throughout the embryo (A). From 24 to 36hpf expression was seen in all formed somites, (B and C). At 36 and 48hpf signals were also found in the neural tube in a ventral to dorsal decreasing gradient (C and E). Strong signals can be detected in the lens (D), in the posterior lateral line ganglion (pl) and in cranial ganglia (cg) (C, E and F). At 72hpf expression was only retained in the anterior dorsal and anterior ventral lateral line ganglion (ad/av), the nucleus of the medial longitudinal fascicle (nucMLF), in reticulospinal neurons of the hindbrain (rsn), and the vagal epibranchial ganglion (v) (G). Embryos are shown in lateral (A–C, E and G) or dorsal view with anterior to the left (D, F and H). Immunohistochemistry with a 3A10- antibody was performed on whole mount in situ hybridizations (I, J and K). Co-labelling of dkk3a(dkk3l) and 3A10-positive neuronal structures by immunohistochemistry (brown staining in I–K) reveal overlapping expression in the nucleus of the medial longitudinal fascicle (nucMLF). Higher magnification of the midbrain in a whole embryo from dorsal (J) and a transversal sections (K) further confirm overlapping expression in the nucMLF. M indicates 3A10 positive Mauthner axons. Scale bars in all figures correspond to 50 μm.
Reprinted from Gene expression patterns : GEP, 11(8), Untergasser, G., Martowicz, A., Hermann, M., Töchterle, S., and Meyer, D., Distinct expression patterns of dickkopf genes during late embryonic development of Danio rerio, 491-500, Copyright (2011) with permission from Elsevier. Full text @ Gene Expr. Patterns