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Fig. 6 Nipbls affect gut and heart development through regulation of endodermal gene expression.
(A, B) Exogenous gata5 and sox32 rescue a subset of gut and heart defects in nipbla/b-morphant embryos. mRNAs for gata5 (10 pg) or sox32 (2.5 pg) were injected into control embryos (no MO; left 3 columns) or nipbla/b-morphants (right 3 columns), and heart (A) and gut (B) morphologies were examined by ISH with cmlc2 (32 hpf) and foxa3 (52 hpf) probes, respectively. Numbers of embryos examined are shown at the top. (C, D) Gut bifurcation, similar to the type II gut phenotype found in nipbla/b-morphants, was induced by simultaneous reduction of sox17 and foxa2. (C) Frequency of gut bifurcation in sox17 and foxa2-single and double-morphants at 52 hpf. (D) Gut/visceral organ morphologies analyzed by ISH with foxa3 probe. View is dorsal, with anterior to the top. Scale bar: 50 μm.