Fig. 2
mRNA expression of the pumilio-2 in adult zebrafish tissues. A Analysis of mRNA expression of the pumilio-2 in several zebrafish tissues using RT-PCR. RNA template and β-actin were used as negative and positive controls, respectively. The tissues shown include the ovary, testis, liver, kidney, heart, muscle and brain. B Expression of the zebrafish pumilio-2 in ovary and testis (In situ hybridization). a: Whole in situ hybridization of ovary; b: Ovary slide following in situ hybridization. The nucleus is located in the center of the oocyte; I?IV: Oocyte during phases I?IV; c: Whole in situ hybridization of testis; d: Testis slide after in situ hybridization; e: Enlarged the black rectangle of d. Scale bars: 0.3 mm in all panels