ZFIN Image: Batut et al., 2011, Fig. S5

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Figure Caption

Fig. S5

CARM1 (C1) and PRMT5 (P5) control myogenesis differentially. (A,B) One-cell stage embryos were injected with the indicated morpholino (Mo) or a control Mo (MoCt) and were analyzed by whole-mount immunohistochemistry for (A) slow fibers and (B) fast fibers at 18-somite stage. Lateral views, anterior to the left. Both slow and fast fiber formation require PRMT5 (n = 12). CARM1 is necessary for fast fiber specification but does not affect slow fiber specification (n = 15). Antibodies used were: F310 fast Myosin Light Chain (DSHB), F59 slow Myosin Heavy Chain (DSHB) and appropriate Alexa Fluor-conjugated secondary antibodies (Molecular Probes, Eugene, OR, US). Nuclei were stained with TO-PRO3 (Molecular Probes, Eugene, OR, US) according to the manufacturer′s protocol.

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