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Fig. S8 Chromogenic analysis confirms that Mnx1 knockdown alters alpha:beta cell ratios in a similar fashion in untreated and RA-treated embryos. Ventral images of 30 hpf embryos. In situ hybridizations for insulin (ins, red) and glucagon (gcga, blue). (A,D,G,J) Untreated embryos, (B,E,H,K) DMSO-treated and (C,F,I,L) RA-treated embryos. Red arrowheads indicate insulin-expressing cells and black arrowheads indicate glucagon-expressing cells in the head endoderm region.
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