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Fig. S2 Protein translation is not required for RA to induce mnx1 expression. (A) Schematic representation of pharmacological treatments. For experiments shown in B and C, embryos were treated with 1 μM RA or 10 μM R115866 an inhibitor of Cyp26 function (Kinkel et al., 2009) for 1 hour, reagents were removed at 9 hpf, and embryos fixed for analysis at 11 hpf. For experiments shown in E-G, embryos were treated with 1 μM RA or with 50 μg/ml CHX (Poulain and Lepage, 2002), or with both RA and CHX together, at 8 hpf for 3 hours until embryos were fixed for analysis at 11 hpf. (B-G) Whole-mount in situ hybridization for mnx1 (blue) and myoD (red). Anterior to left.