ZFIN Image: Smith et al., 2011, Fig. 6

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Figure Caption

Fig. 6

Bmp and Nodal induce lefty1 independently.

(A–D) Tg(hsp70l:bmp2b) embryos were left at 28°C (A,C) or heat-shocked at 10 hpf to induce bmp2b expression (B,D). A subset of embryos were incubated in the presence of the Nodal inhibitor SB431542 directly after the heat-shock. Embryos were analysed by in situ hybridisation for lefty1 expression at 15-somites. (E–H) Tg(hsp70l:nog3) embryos were left at 28°C (E,G) or heat-shocked at 10 hpf to induce noggin3 expression (F,H). In a subset of embryos a bead preincubated with recombinant Nodal was placed in the ALPM. Embryos were analysed by in situ hybridisation for lefty1 expression at 18-somites. All embryos are shown as lateral views with dorsal to the right and anterior to the top. Arrows point to most anterior lefty1 expression domain. Numbers in lower right represents the number of embryos that displayed the phenotype represented in the panels.

Figure Data

Expression / Labeling details

Acknowledgments

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