Fig. 10

Fig. 10 myl7 at 22s in (A) control, (B) DEAB treated, (C) 2 ng fgf8a MO mixture injected, and (D) DEAB treated with 2 ng fgf8a MO mixture injected embryos. (B) Expression of myl7 is increased in DEAB treated embryos. (C) Expression of myl7 is reduced in embryos injected with the 2 ng fgf8a MO mixture. (D) Expression of myl7 is rescued in embryos treated with DEAB and injected with 2 ng fgf8a MO mixture. (E) Area of total cells expressing myl7 in control, DEAB treated, fgf8a MO mixture injected, and DEAB treated plus 2 ng fgf8a MO mixture injected embryos. See Supplemental Table 3. Asterisks indicate statistically significant difference from sibling control embryo measurements (p < 0.05) using Student′s t-test. Single experiments that examined heart and forelimb phenotypes in sibling embryos and experiments that only examined heart or forelimb phenotypes gave equivalent results (not shown). Images are dorsal views with anterior up.

Inhibition of Fgf8a function can rescue cardiac differentiation marker expression in RA signaling-deficient embryos. Expression of myl7 at 22s in (A) control, (B) DEAB treated, (C) 2 ng fgf8a MO mixture injected, and (D) DEAB treated with 2 ng fgf8a MO mixture injected embryos. (B) Expression of myl7 is increased in DEAB treated embryos. (C) Expression of myl7 is reduced in embryos injected with the 2 ng fgf8a MO mixture. (D) Expression of myl7 is rescued in embryos treated with DEAB and injected with 2 ng fgf8a MO mixture. (E) Area of total cells expressing myl7 in control, DEAB treated, fgf8a MO mixture injected, and DEAB treated plus 2 ng fgf8a MO mixture injected embryos. See Supplemental Table 3. Asterisks indicate statistically significant difference from sibling control embryo measurements (p < 0.05) using Student′s t-test. Single experiments that examined heart and forelimb phenotypes in sibling embryos and experiments that only examined heart or forelimb phenotypes gave equivalent results (not shown). Images are dorsal views with anterior up.