ZFIN Image: Sorrell et al., 2011, Fig. 9

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Figure Caption

Fig. 9

Moderate reduction of Fgf8a can rescue forelimb development in RA signaling-deficient embryos. (A) Forelimb (pectoral fin) of control sibling embryo. (B) Representative embryo treated with DEAB lacks forelimbs. (C) Representative embryo treated with DEAB and injected with 2 ng fgf8a MO mixture that has forelimbs. Views are lateral at 72 hpf. (D) Graph indicating the percentage of control sibling (n = 27) embryos, DEAB treated embryos (n = 34), and DEAB treated embryos that were also injected with 2 ng fgf8a MO mixture (n = 21) with forelimbs from a representative experiment. 100% (n = 13) of embryos injected with 2 ng fgf8a MO mixture had forelimbs (not shown). Injection of a 6 ng fgf8a MO mixture into DEAB treated embryos also restored fins as 63% (10 had forelimbs; n = 16).

Acknowledgments

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Reprinted from Developmental Biology, 358(1), Sorrell, M.R., and Waxman, J.S., Restraint of Fgf8 signaling by retinoic acid signaling is required for proper heart and forelimb formation, 44-55, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.