Fig. 3

Fig. 3 Increased Fgf signaling expands early cardiac differentiation markers. (A–F) myl7, vmhc and amhc expression in heat-shocked control sibling embryos and Tg(hsp70:ca-fgfr1)^pd3 embryos. (A) myl7, (C) vmhc and (E) amhc expression in heat-shocked control sibling embryos. The amount of cells expressing (B) myl7, (D) vmhc, and (F) amhc is increased in Tg(hsp70:ca-fgfr1)^pd3 embryos with increased Fgf signaling at TB stage. (G) Graph indicating the fold difference in total area of cells expressing myl7, vmhc and amhc from heat-shocked control sibling and Tg(hsp70:ca-fgfr1)^pd3 embryos at the TB and 8s stages. For area measurements, see Supplemental Table 2. Asterisks indicate that there was a statistically significant difference in the populations used for these fold comparisons (Supplemental Table 2). Images of ISH in A–F are dorsal views with anterior up.

Increased Fgf signaling expands early cardiac differentiation markers. (A–F) my17, vmhc and amhc expression in heat-shocked control sibling embryos and Tg(hsp70:ca-fgfr1)^pd3 embryos. (A) my17, (C) vmhc and (E) amhc expression in heat-shocked control sibling embryos. The amount of cells expressing (B) my17, (D) vmhc, and (F) amhc is increased in Tg(hsp70:ca-fgfr1)^pd3 embryos with increased Fgf signaling at TB stage. (G) Graph indicating the fold difference in total area of cells expressing my17, vmhc and amhc from heat-shocked control sibling and Tg(hsp70:ca-fgfr1)^pd3 embryos at the TB and 8s stages. For area measurements, see Supplemental Table 2. Asterisks indicate that there was a statistically significant difference in the populations used for these fold comparisons (Supplemental Table 2). Images of ISH in A–F are dorsal views with anterior up.