ZFIN Image: Sorrell et al., 2011, Fig. 1

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Figure Caption

Fig. 1

Increased Fgf signaling in the post-gastrula embryo eliminates forelimbs. (A,B) Representative images from a heat-shocked control sibling embryo and a Tg(hsp70:ca-fgfr1)^pd3 embryo. (A) Heat-shocked control sibling embryos had forelimbs (yellow outline). (B) Forelimbs are absent in Tg(hsp70:ca-fgfr1)^pd3 embryos heat-shocked at the TBstage. (C) Graph indicating the percentage of Tg(hsp70:ca-fgfr1)^pd3 embryos with forelimbs when heat-shocked at the TB (n = 10), 8s (n = 15) or 14–16s (n = 6) stages from a representative experiment. 100% of control sibling embryos heat-shocked at the TB (n = 11), 8s (n = 16) or 14–16s (n = 7) stages had forelimbs. (D) Graph indicating the percentage of DEAB treated embryos with forelimbs when treatments were initiated at the TB (n = 42), 8s (n = 46) and 14–16s (n = 27) stages. 100% (n = 85) of untreated control sibling embryos had forelimbs.

Acknowledgments

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Reprinted from Developmental Biology, 358(1), Sorrell, M.R., and Waxman, J.S., Restraint of Fgf8 signaling by retinoic acid signaling is required for proper heart and forelimb formation, 44-55, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.