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Fig. S7 RT-PCR analysis of the expression of Wntreceptors and downstreamcomponents of the canonicalWntsignaling pathway. Expression of theWntreceptors (Fzd1, Fzd2, Fzd3, Fzd3L, Fzd4, Fzd6, Fzd7a, Fzd7b, Fzd8a, Fzd8b, Fzd9, Fzd10, ROR2, RYK) and of downstreamcomponents (APC, Axin1, Axin2, DVL2, DVL2L, DVL3, GSK3b) analyzed by RT-PCR at one- to two-cell stage (0.5 h), 1,000-cell stage (3 h), sphere stage (4 h), 40% epiboly (5 h), 60% epiboly (7 h), and at 24 hpf.
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