Fig. 4 Effects of injection of cdh2 morpholino antisense oligonucleotides (cdh2MO) on the development of the pectoral limb buds. A,B: Lateral views of the pectoral limb bud of whole-mount embryos stained with an anti-Hu antibody (Sigma). This antibody labels differentiating neurons (Marusich et al., 1994). The staining in the limb buds, although nonspecific, makes their visualization easier. Dorsal is up and anterior is to the left. C–E: Cross-sections of limb buds (dorsal up) stained with Alcian blue showing anatomy of control (C) and cdh2MO-injected (D,E) embryos. ed?, possible endoskeleton; Lb, limb bud; Other abbreviations are the same as in Figure 1.
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