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Fig. S4 Hair cell polarities in both ventral maculae of heat-shocked hsp70:fgf3 embryos resemble those in the anterior macula of wild-type embryos. (A-N) Maculae of hsp70:fgf3 embryos heat-shocked at 10S, and stained at 86 hpf with anti-acetylated tubulin antibody to mark the kinocilia (red) and FITC-phalloidin to mark the actin rich stereocilia (green) of each hair cell. (A-C,H-J) Confocal z-stacks showing overlaid tubulin and phalloidin stains. B,C,I,J show enlarged views of the anterior and posterior maculae of the ears shown in A and H. (D,E,K,L) Single channel z-stacks showing phalloidin stain only. (F,G,M,N) Polarity maps obtained from the phalloidin/tubulin stained specimens. Orientations shown by arrows: a, anterior; l, lateral.