ZFIN Image: Hammond et al., 2011, Fig. S3

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Figure Caption

Fig. S3 Expression of medial and ventral otic markers in heat-shocked hsp70:fgf3, SU5402-treated wild-type and SU5402-treated smo^-/- embryos. Phenotypically wild-type sibling and smo^-/- mutant embryos were treated from 10 to 20S with 10 μM SU5402/0.5% DMSO. Control sibling and smo^-/- mutant embryos were treated with 0.5% DMSO from 10 to 20S. hsp70:fgf3 transgenic and sibling embryos were heat-shocked at 10S. (A-F) In situ hybridisation to eya1, a marker of ventral otic territories, in 24 hpf embryos. (G-L) In situ hybridisation to pax2a, a marker of medial otic territories, in 25S embryos. Both eya1 and pax2a are expressed in their normal otic domains in all treated and heat-shocked embryos; however, levels of both are severely reduced in otic vesicles of SU5402-treated smo^-/- embryos. A-F: Lateral views; anterior to left, dorsal to top. G-L: Dorsal views; anterior to left. Both ears are shown. Scale bars: 50 μm for all panels in a row.

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