|
Fig. 4 Heat shock of hsp70:fgf3 embryos at 10S leads to a complete loss of posterior otic domains and a mirror image duplication of anterior otic structures. (A-C) Ears of live 68 hpf hsp70:fgf3 and control wild-type zebrafish embryos heat-shocked at 10S. (D-L) Confocal z-stacks of 4 dpf ears stained with FITC-phalloidin to mark sensory hair cells. (D-F) Lateral focal planes showing the maculae. (G-I) Medial focal planes showing the cristae. (J-L) Dorsal views. (M-O) In situ hybridisation to otx1. (P) The macula of an 86 hpf heat-shocked hsp70:fgf3 embryo stained with anti-acetylated tubulin antibody (kinocilia; red) and FITC-phalloidin (stereocilia; green). (Q) Hair cell polarity maps obtained from the maculae shown in P. A-I: Lateral views; anterior to left, dorsal to top. J-Q: Dorsal views; anterior to left, medial to top. am, anterior macula; ao, anterior otolith; c, cristae; pm, posterior macula; po, posterior otolith. Arrow in I marks the lateral crista, which is enlarged. Scale bars: 50 μm.