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Fig. 1 Anterior otic character is reduced in lia-/- (fgf3-/-) homozygotes. (A-F) Live 72 hpf lia-/- and sibling (sib) zebrafish inner ears. (G-L) Confocal z-stacks of 84 hpf ears stained with FITC-phalloidin to mark sensory hair cells. (M) The anterior macula of a 5 dpf lia-/- embryo stained with anti-acetylated tubulin antibody (kinocilia; red) and FITC-phalloidin (stereocilia; green). (N,O) Typical polarity maps for wild-type maculae. (P) Hair cell polarity map obtained from the specimen shown in M. (Q,R) Polarity maps from two further lia-/- specimens. A,B,D,E,G,H,J,K: Lateral views; anterior to left, dorsal to top. A,D,G,J: Lateral focal plane. B,E,H,K: Medial focal plane. C,F,I,L: Dorsal views; anterior to left, medial to top. am, anterior macula; ao, anterior otolith; c, cristae; pm, posterior macula; po, posterior otolith. Asterisks indicate semicircular canal pillars. Scale bars: 50 μm.