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Fig. S2 Early neurogenesis in cug2 morphant embryos. A, A′. neurogenin1 (ngn1) expression in control MO- (A) and cug2 MO-injected (A′) embryos at the 3-somite stage (3 ss). The number of ngn1-positive neuronal precursors is increased at neural plate in cug2 MO-injected embryos (80%/n = 20). B, B′. Expression of deltaA (dlA) in control MO- (B) and cug2 MO-injected (B′) embryos at 3 ss. cug2 MO-injected embryos show increase of delta A-expressing neuronal precursor (70%/n = 20). C, C′. huC expression in control MO- (C) and cug2 MO-injected (C′) embryos at 3 ss. The number of huC-positive differentiating neurons is decreased in cug2 MO-injected embryos (77%/n = 26). D-F′. At the 20-somite stage (20 ss), ngn1, deltaA, and huC expression in control MO- (D, E, F) and cug2 MO-injected embryos (D′, E′, F′). During secondary neurogenesis (20 somite-stage), both neuronal precursors (ngn1, delta A) and differentiating neurons (huC) are decreased in cug2 MO-injected embryos. G, G′. Dorsal view at 24 hpf. The number of huC-positive differentiating neurons is dramatically decreased in cug2 morphants (G′). H. Quantification of delta A, ngn1, and huC-positive cells in control and cug2 MO-injected embryos at 3-somite stage in the area indicated in A-C′ (n = 10).