ZFIN Image: Lo et al., 2011, Fig. 5

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Figure Caption

Fig. 5

Effect of IGFBP-1 knockdown on PGC migration in zebrafish embryos.

(A and B) Embryos were injected with GFP-nosl 32UTR mRNA alone and subsequently exposed to either normoxia or hypoxia. PGC migration was impaired in hypoxic embryos (B) as compared with normoxic embryos (A). (C and D) Embryos were co-injected with GFP-nosl 32UTR mRNA and IGFBP-1 MO and subsequently exposed to either normoxia or hypoxia. (D) PGC migration defect in hypoxic embryos was minimized by IGFBP-1 MO knockdown. Scale bar: 200 μm. (E) Mean number of mis-migrated ectopic PGCs in normoxia and hypoxia embryos injected with GFP-nosl 32UTR mRNA alone or co-injected with GFP-nosl 32UTR mRNA and IGFBP-1 MO. The mis-migrated ectopic PGCs phenotype could be rescued by IGFBP-1 MO knockdown as indicated by reduction in number of mis-migrated ectopic PGCs in hypoxic embryos. * denotes p<0.05.

Acknowledgments

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