ZFIN Image: Eames et al., 2011, Fig. S1

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Figure Caption

Fig. S1

Exogenous wild-type fam20b expression does not alter skeletal phenotypes of wild types or xylt1^b1128 mutants, but can rescue cartilage of fam20b^b1125 mutants with a single induction at 55 hpf. A–E, dissected, flat-mounted pharyngeal skeletons of Alcian blue/Alizarin red-stained (A,B) and Alcian blue-stained (C–E) larvae. Injection of larvae with wild-type fam20b under control of the beta-actin2 promoter did not alter skeletal phenotypes of wild types (A) or xylt1^b1128 mutants (B). Injection of larvae with wild-type fam20b under control of the hsp70l promoter rescued cartilage matrix production in fam20b^b1125 mutants when heat shocked at 55 hpf (E), compared to injected fam20b^b1125 embryos that did not undergo heat shock (D), while heat shock had no effect on uninjected control larvae (C). Abbreviations: HS = heat shock; UIC = uninjected control.

Acknowledgments

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