|
Fig. 7
Thyroid hormone partially mediates PTU effect on neural crest.
96 hpf roy Tg(sox10::EGFP) embryos were treated with 100 nM T3/100 nM T4 and 0.003% (E?H) or 0.03% (I?L) PTU at 6 hpf or 22 hpf. High concentrations of T3 and T4 in the absence of PTU caused thickening of jaw cartilage and frontal bossing (A?D)) when added at 6 or 22 hpf. Treatment with 0.003% PTU lessened the effects of exogenous T3 and T4 (E?H compared to I?L). 100 nM T3/100 nM T4 improved, but did not restore the neural crest defects induced by treatment with 0.03% PTU at 6 (I, J compared to Figure 5I, L) or 22 hpf (K, L compared to Figure 5M, P). PA, pharyngeal arches.