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Fig. S10

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ZDB-IMAGE-110909-44
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Figures for Johnson et al., 2011
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Figure Caption

Fig. S10

Proliferation within the arches is not affected in vgll2a morphants. Three-dimensional projections of confocal slices of representative uninjected (A,C,E) and vgll2a morphant embryos (B,D,F) at 24 (A,B) 48 (C,D) and 72 hpf (E,F) stained with zn-8 (anti-Alcama) and anti-phospho-histone H3 antibodies in lateral views, and quantification of cell death in uninjected and morphant embryos. (A, C, E) Uninjected and (B, D, F) morphant embryos injected with 5 ng vgll2a ATG MO exhibit a similar number of proliferating cells (red) per 100 μm3 within arches volumes modeled within the white dashed lines based on zn-8 staining (green) relative to uninjected controls, at 24, 48 and 72 hpf. (G) Quantification of the average number of phospho-histone H3 positive cells / 100 μm3 of arch volume per embryo. Error bars represent standard error of the mean. Numbers at the base of each bar represent the number of embryos counted for each condition. Scale bar = 100 μm.

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Reprinted from Developmental Biology, 357(1), Johnson, C.W., Hernandez-Lagunas, L., Feng, W., Melvin, V.S., Williams, T., and Artinger, K.B., Vgll2a is required for neural crest cell survival during zebrafish craniofacial development, 269-81, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.