ZFIN Image: Johnson et al., 2011, Fig. 5

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Figure Caption

Fig. 5

Cell death within the pharyngeal arches is increased in vgll2a morphants. Three-dimensional projections of confocal slices of representative uninjected (A,C,E) and vgll2a morphant embryos (B,D,F) at 24 (A,B) 48 (C,D) and 72 hpf (E,F) stained with zn-8 (anti-Alcama) and anti-activated caspase 3 antibodies in lateral views, and quantification of cell death in uninjected and morphant embryos (G). Embryos injected with 5 ng vgll2a ATG MO (B,D,F) exhibit a greater number of activated caspase 3 positive cells (red) per μm³ within arches volumes modeled within the white dashed lines based on zn-8 staining (green) relative to uninjected controls (A, C, E), at 24, but not 48 or 72 hpf. (G) Quantification of the average number of active caspase 3 positive cells / 100 μm³ of arch volume per embryo (Student′s T Test, **: p < 0.01). Error bars represent standard error of the mean. Numbers at the base of each bar represent the number of embryos counted for each condition. Scale bar = 100 μm. Anterior is to the left in panels A-F.

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Acknowledgments

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Reprinted from Developmental Biology, 357(1), Johnson, C.W., Hernandez-Lagunas, L., Feng, W., Melvin, V.S., Williams, T., and Artinger, K.B., Vgll2a is required for neural crest cell survival during zebrafish craniofacial development, 269-81, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.