IMAGE

Fig. 6

ID
ZDB-IMAGE-110816-15
Antibodies
Source
Figures for Mapp et al., 2010
Image
Figure Caption

Fig. 6 Centrosome remains primed for tangential migration in Pk1b-deficient neurons. A-D: Dorsal views of fixed embryos illustrating positioning of centrosome in migrating neurons. A: Control, 18 hpf. B: Pk1b morphant, 18 hpf. C: Control, 24 hpf. D: Pk1b morphant, 24 hpf. Red: zCREST1:membRFP transgene expression. Blue: EphA4 immunostaining, to label r3 and r5. Green: Υ-tubulin immunostaining, which allows visualization of centrosomes (bright puncta; white arrowheads) as well as the microtubule network (fainter green staining). Region of each cell with no green staining corresponds to nucleus. Flattened confocal z-stacks. Scale bars = 50 μm. A-D: Higher magnification views of regions denoted by yellow boxes in A-D. E: Schematic demonstrating measurement of centrosome position. A bisecting line was drawn from the centrosome (bright green puncta) through each neuron. The angle of this line, relative to the A-P axis was defined as the centrosome position. F-M: Rose diagrams illustrating the range of centrosome positions in migrating FBMNs. Numbers along right side indicate scale of y-axis (i.e., percentage of total neurons for each angle subset). n values indicated represent total number of neurons scored for each class. N = 5 embryos for each class.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.