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Fig. S3
netrin 1a expression at the HMS is abolished in embryos treated with 50 ยตM cyclopamine. Lateral view of in situ hybridizations at 32 hpf. (A) In 1% DMSO-treated controls, netrin 1a is expressed in the neural tube (NT) and muscle pioneers (MP) at the HMS (arrows). (B) netrin 1a is expressed in the NT but lost at the HMS (red arrowhead) in embryos that lack MPs following cyclopamine treatment. NT, neural tube; MP, muscle pioneer. Scale bar: 100 μm.
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