Fig. S9
DFC-specific knockdown of cnpy1 attenuates F-actin accumulation at the cell-cell contact sites of DFCs. (A, B) Distribution of F-actin (red) and FITC-labeled MO (green) in DFCcontrol-MO (A) or DFCcnpy1-MO (B) embryos. Dorsal view, anterior to the top. Scale bar: 20 μm. White dotted lines mark the plasma membrane outlines of MO-containing DFC populations. (A’, B’) Higher-magnification images highlight MO-containing DFCs. In control-MO-containing DFCs (A’), F-actin accumulated at the cell-cell contact sites of the plasma membrane. However, accumulation of F-actin was limited in cnpy1-MO-containing DFCs (B’).