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Fig. S3
Reprogramming of cardiac and haemangioblast cells by Fgf signalling is mediated by lycat and nkx2.5. (A) Quantitative PCR on cDNA obtained from RNA extracted from the heart region of 36 hpf DMSO- and SU5402-treated embryos assayed for pu.1, kdrl and cmlc2 expression normalized to gapdh. Shown are fold changes in expression relative to the DMSO control, from one representative experiment. Four biological replicates were analysed in triplicate. Error bars indicate s.e.m., asterisks indicate statistically significant differences (P<0.0005) compared with DMSO controls. (B) cmlc2::GFP transgenic fish at 48 hpf show an overall heart size increase when injected with scl+etsrpMO. The increase is even greater in scl+etsrp morphants that have been co-injected with nkx2.5 mRNA. Dashed lines outline the atrium (red) and the ventricle (yellow). Scale bar: 100μm.