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Fig. 7
Role of cathepsin D in eye development.
Hematoxylin-eosin staining of eye sections derived from 4 dpf micro-injected larvae (CTRL = control injections; S-MPO = splicing morpholino; T-MPO = translation morpholino; RESCUED = translation morpholino plus 200 pg/egg of mutant CD mRNA). Images at high magnification clearly show the palisade of microvilli of RPE cells (which contain melanin granules, MG) that interdigitate in the layer of photoreceptor cells (PRC) in CTRL, S-MPO and RESCUED zebrafish, while this is completely absent in T-MPO CD KD zebrafish (note the RPE cells containing melanine granules close to the PRC layer). Scale bar is 20 μm. Images representative of five (three for Rescued) independent experiments.