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Fig. S1
Flatten embryo and count DA neurons. Take embryos hybridized with dat probe as an example. After in situ hybridization, transfer embryos into glycerol and equilibrate for 10 min. Then put it on a slide and flatten it softly with a cover slip to disperse the neurons (A, dosal view; B, lateral view). C and D show the same embryo before and after being flattened.
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