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Fig. S3
Localization of fascin 2b and espin proteins in fixed COS-7 cells. Schematics of the proteins expressed in cells are portrayed: GFP-WT fascin 2b (A), GFP (B), GFP-espin (C), and GFP-WT fascin 2b and mCherry-espin (G). To visualize actin-based filopodia, cells were labeled with Alexa 568 phalloidin (red)(D-F) or Alexa 633 phalloidin (blue) (J,K). Merged images show localization of GFP-WT fascin 2b (green) (D) or GFP-espin (green) (F) to filopodia where they overlap with actin (yellow). No filopodia are detectable in a fixed cell that expresses only GFP (green) (E). Coexpression of GFP-WT fascin 2b (H) and mCherry-espin (I) shows that both proteins colocalize (white) (K) to phalloidin-labeled filopodia (J) in a fixed COS-7 cell. Scale bars are 5 μm.