ZFIN Image: Ikenaga et al., 2011, Fig. 3

Image

Figure Caption

Fig. 3

Homozygous embryos are Pax8 hypomorphs. A: The exon composition of zebrafish pax8 indicating the insertion site of Tol2. Primers for the qPCR were designed so that the amplicon spans the junction between exon 5 and exon 6. B: qPCR of the pax8 transcript in wild-type (wt), pax8^+/m, and pax8^m/m fish at 1 dpf and 10 dpf. Wild type at 1 dpf was used as a reference sample to obtain relative transcript amounts. Error bars represent the SEM of the average from triplicates of a single run (n = 3). C,D: Confocal images of RFP signals in pax8^m/m fish at 24 hpf (C) and 4 dpf (D). E–G: The gross morphology of larva at 4 dpf. E is wild type, F is pax8^m/m, and G is pax2a^–/–. The pax2a^–/– larva is deformed and dies at 7–10 dpf. H,I: The brain morphology is normal in the pax8^m/m larva (I) compared with the WT larva (H). Top is the lateral view and bottom is the dorsal view. J: Adult pax8^m/m fish. Scale bars = 200 μm in C,D; 1 mm in G (applies to E–G; 500 μm in I (applies to H,I).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Comp. Neurol.