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Fig. s6
RAR signaling regulates gene expression in the VR/OS and in POM. Lateral views of heads of 31-hpf embryos treated with DMSO or 10 μM AGN from 3s and hybridized with the indicated probes. (A) AGN treatment causes down-regulation of vax1, net1a, cyb5a, and itgA5 in VR/OS cells (arrowheads). Triangles point to vax1 expression in the ventral forebrain. (B) In the control eye, dhrs3a is expressed in VR/OS cells (arrowhead), in the dorsal retina (triangle), and in dorsal POM (arrow). All these domains are down-regulated in the AGN-treated embryo. AGN treatment decreases foxC1a and nlz1 expression in both anteriorventral (arrowheads) and anterior-dorsal (triangles) POM as well as twist1a expression in dorsal POM only (triangles). (C) AGN treatment decreases eya2, pitx2, and lmx1b1 expression in anterior-ventral (arrowheads) and/or anterior-dorsal (black triangles) POM. Expression of lmx1b1 within the choroid fissure is also diminished (white triangles). pitx2 is strongly expressed in extraocular mesodermal tissue in both the controls and the AGN-treated embryo (arrows).