Fig. 3

Fig. 3

Lineages Do Not Transfate in the Regenerating Zebrafish Fin

(A) Labeled melanocyte/xanthophore clone, shown at 20 days postamputation (dpa), only regenerated melanocytes and xanthophores. Arrows and arrowheads point out the labeled melanocytes and xanthophores proximal (left) and distal (right) to the amputation plane (dashed line), respectively. The empty white arrow points to an unlabeled melanocyte, whereas the empty yellow arrowhead points to an unlabeled xanthophore. A GFP-labeled melanocyte appears as a black circle surrounded by a green halo (A′), whereas a GFP-labeled xanthophore appears as a yellow circle surrounded by a green halo (A′′). Light-gray line outlines the fin ray.

(B) Intraray glia clone (arrows) only regenerates label in the intraray nerve. Asterisk denotes autofluorescence from xanthophores.

(C) A lateral line clone regeneration at 20 dpa. Arrows and arrowheads point to the lateral line neuromasts and glia proximal (left) and distal (right) to the amputation plane, respectively.

(D) Epidermis (skin) clone only regenerated epidermis.

(E–J) Confocal images (sagittal view) of a regenerating osteoblast clone (E–G), and a dermal fibroblast clone (H–J) at three positions: proximal to amputation plane (E and H), at the amputation plane (F and I), and distal to the amputation plane (G and J). Note that both clones are mosaic in the fin ray, with the osteoblast clone forming a continuous sheet immediately adjacent to medial and lateral surfaces of the bone matrix, in this case labeling the dorsal (top) of the ray, whereas the dermal fibroblast clone labels a population of loose mesenchymal cells in the interior of the fin ray. Dermal fibroblast clones are typically intermixed with unlabeled dermal fibroblasts throughout the intraray space. Red shows alizarin red staining of the bone matrix. Asterisk denotes autofluorescence from xanthophores.

(K) Whole-mount stereoscope view of regenerating vasculature clone; note that only vasculature carries label in the regenerate. Green shows GFP from labeled clone in live fish. Dashed lines indicate amputation plane. Scale bars, 0.2 mm. See also Figure S1.