Expression of flt1 isoforms in zebrafish embryos. (A) Structural organization of membrane-bound flt1 (mflt1) and soluble flt1 (sflt1). sflt1 contains a unique mRNA sequence, not present in mflt1, that encodes the 5′ region of intron 10. (B) Taqman analysis shows higher expression of sflt1 than mflt1 in control embryos at 24 hpf, 30 hpf and 48 hpf. f.c., fold change relative to mflt1 expression at indicated time-points. Error bars indicate s.e.m. (200 embryos/group from four separate experiments). (C) Whole-mount in situ hybridization with antisense riboprobes directed against mflt1 or sflt1; embryos at 30 hpf. Note that mflt1 is expressed in segmental sprouts, aorta and cardinal vein; sflt1 is expressed in segmental sprouts and aorta. Ao, dorsal aorta; PCV, posterior cardinal vein; Se, segmental artery.
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