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Fig. 6 Effects of temperal inhibition of Wnt signaling on swimbladder mesothelium development.
(A, B) Expression of elovl1a in outer mesothelium at 48 hpf and 72 hpf when hs:Dkk1-GFP fishes were heat-shocked from 12 hpf. The elovl1a expressing cells were disorganized at 72 hpf when larvae were heat-shocked from 24 hpf (C), but were well organized with smaller size when larvae were heat-shocked from 30 hpf (D). (E) Expression of elovl1a in wild-type outer mesothelium. The elovl1a expressing cells in the outer mesothelium at 72 hpf were absent when hs:ΔTcf-GFP larvae were heat-shocked from 30 hpf (F), were present but disorganized when larvae were heat-shocked from 42 hpf (G), and were properly organized but with smaller size when fishes were heat-shocked from 42 hpf (H). All embryos were laterally oriented with anterior to the left unless specified. Red arrows indicated absence of swimbladder at putative locations. Abbreviations: g, gut; sb, swimbladder. Scale bar in (A) = 200 μm for all panels.