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Fig. 4 Effects of temporal inhibition of wnt signaling on the epithelium development of swimbladder.
(A–L) Expression of marker genes in hs:Dkk1-GFP embryos after heat-shock treatment at various stages. (A–D) Expression of sox2 in swimbladder epithelium in wild type sibling at 72 hpf (A) and in hs:Dkk1-GFP embryos heat-shocked from different developmental stages as indicated (B–D). Expression of sox2 in the swimbladder epithelium anlage at 30 hpf when heat-shocked from 12 hpf (B). (E–H) Expression of hb9 at 72 hpf in wild type swimbladder epithelium, in 72 hpf hs:Dkk1-GFP larvae heat-shocked from 8 hpf, 30 hpf (G) and 36 hpf (H). (I, J) Expression of foxA1 at 72 hpf in the epithelium of endoderm organs in wild type and transgenic larvae heat-shocked from 12 hpf (J). (K, L) Expression of gata6 in the epithelium of endoderm organs at 72 hpf in wild type and transgenic fishes heat-shocked from 12 hpf. (M–P) Expression of hb9 in the epithelium of swimbladder at 72 hpf in transgenic larvae heat-shocked from 30 hpf, 36 hpf, 42 hpf and 48 hpf. Note the presence of anterior swimbladder bud (asb) in (D, H, P). All embryos were laterally oriented with anterior to the left unless specified. Abbreviations: asb, anterior swimbladder bud; ex, exocrine pancreas; g, gut; li, liver; n, notochord; pd, pneumatic duct; ph, pharynx; pi, pancreatic islet; sb, swimbladder. Scale bar in (A) = 200 μm for all panels.