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Fig. 8 Effects of Wnt inhibition on cell apoptosis in swimbladder.
hs:ΔTcf-GFP heterozygous larvae and their wild type siblings were heat-shocked at 66 hpf and fixed at 72 hpf for TUNEL assay. (A–F) TUNEL-positive cells (red) and DAPI-stained cells (green) shown in cross section. The number of TUNEL-positive cells in swimbladder greatly increased in transgenic fishes (D–E) (n = 11 of 13, 85%) compared to that of controls (A–C) (n = 9 of 10, 90%). (G, H) Imaging of whole mount TUNEL-stained (red) transgenic fishes (H) and controls (G). (I) The statistics assays showed that the percentage of apoptosis cells in heat-shocked transgenics increased from 1.45% to 9.26% (I) (p = 0.01). Note the globally increased number of TUNEL-positive cells in (H). All embryos were laterally oriented with anterior to the left. Dotted white circles indicated swimbladder. Abbreviation: g, gut. Scale bar = 200 μm for all panels.