ZFIN Image: Herpin et al., 2010, Fig. 7

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Figure Caption

Fig. 7

(A) Testis-specific GFP expression in 9KbDmrt1bYprom::GFP transgenic fish. (B) From testis formation (10 dph) up to adulthood, in 9KbDmrt1bYprom::GFP transgenic fish, robust testicular GFP expression is persistently noticeable in the epithelial cells of the intratesticular efferent duct. (C,D) Concomitantly, in agreement with the endogenous dmrt1bY expression, from 10 dph up to 30?35 dph strong specific GFP fluorescence is also detected in Sertoli cells. (E?G,I,J) By 35 dph, Sertoli cell-specific decline in fluorescence (mCherry) could be observed (E,I,H). In double transgenic fish (9KbDmrt1bYprom::mCherry and BACdmrt1a::GFP) this 9KbDmrt1bYprom-driven mCherry decline in expression is balanced by the rise of dmrt1a expression in Sertoli and germ cells (GFP in F,G,J). (H?J) In fully mature testes (after 45?50 dph), 9KbDmrt1bYprom-driven mCherry expression remains in scattered Sertoli cells around the germ cells (GFP in N) while Dmrt1a is now predominantly expressed in Sertoli cells (J).

Acknowledgments

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