Fig. 1
The dlx5/6:GFP transgene labels specific neuronal populations in the larval zebrafish brain. (A) A single confocal section acquired 40 μm below the dorsal surface of the brain. GFP transgene expression is observed in cells in the optic tectum (OT) and cerebellum (CB). (B) At a depth of 80 μm dense GFP labeling of cells is seen in the olfactory bulb of the telencephalon (T), in addition to sparse labeling throughout the diencephalon (D) and mesencephalic structures such as the torus semicircularis (TS). (C) At a depth of 120 μm labeling is largely restricted to the telencephalon and the ventral thalamus (VT). Dashed line indicates midbrain–hindbrain boundary. Scale bar, 50 μm.