ZFIN Image: Kikuchi et al., 2011, Fig. 2

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Figure Caption

Fig. 2

Induction of raldh2 Expression in Various Injury Models

(A and B) Stab injuries into the ventricular apex assessed for raldh2 induction (A) and fli1:EGFP expression (B) at 7 days poststab (dps). Arrows, needle entry site. Scale bar represents 100 μm.

(C) Confocal image of Raldh2 immunofluorescence in fli1:EGFP⁺ endocardial cells with rounded morphology at the injury site (arrowheads). Scale bar represents 20 μm.

(D–H) raldh2 induction after intraperitoneal LPS or vehicle (PBS) injection. Scale bar represents 100 μm (D–L).

(I–L) Endocardial raldh2 (J), hand2 (K), and gata5 (L) expression surrounding spontaneous infarcts (asterisks) within cultured ventricular explants. Dead cardiac tissue was identifiable by the absence of cell nuclei (I).

Acknowledgments

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Reprinted from Developmental Cell, 20(3), Kikuchi, K., Holdway, J.E., Major, R.J., Blum, N., Dahn, R.D., Begemann, G., and Poss, K.D., Retinoic Acid production by endocardium and epicardium is an injury response essential for zebrafish heart regeneration, 397-404, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell