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Fig. 2

ID: ZDB-IMAGE-110322-17
Publication: Laux et al., 2011 - Dynamic analysis of BMP-responsive smad activity in live zebrafish embryos
All Figures: Figures for Laux et al., 2011

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Fig. 2

Expression of the bre:egfp transgene is responsive to changes in BMP type I receptor signaling. Embryos were left uninjected (a–c) or injected with 5 pg alk1^CA mRNA (d–f) or 100 pg alk5^CA mRNA (g–i) at the one- to two-cell stage and assayed at shield stage (6 hpf). a, d, g: Expression of bre-driven EGFP fluorescence in live embryos. b, e, h: Expression of bre-driven egfp mRNA assayed by in situ hybridization. c, f, i: Expression of pSmad1/5/8 assayed by immunofluorescence. Inset in f is pSmad1/5/8 (green)/DAPI (magenta) merge. Lateral views, animal pole up, dorsal right. Original magnification 80× except inset, 200×.

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