ZFIN Image: Mickoleit et al., 2011, Fig. 4

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Figure Caption

Fig. 4

Figure 4. Dnd counteracts the miR430-mediated inhibition of hub expression in PGCs. A: Dnd knockdown results in reduced expression of the Venus protein in PGCs of embryos injected with Venus.hub 32UTR RNA (top right) compared to the expression observed in control morpholino-treated embryos (top left). This effect is not observed for a CFP-based reporter containing the hub 32UTR with the mutated miR430 seed (compare bottom panels). B: Signal intensity measurements at 8hpf reveal a significantly reduced Venus expression. The signal is depicted as the ratio between the Venus and the CFP signals. n is the number of analyzed PGCs. Error bars show SEM. ***P < 0.001 in t-test.

Acknowledgments

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