ZFIN Image: Collery et al., 2011, Fig. 1

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Figure Caption

Fig. 1

Figure 2. Expression of bone morphogenic protein response element: destabilized enhanced green fluorescent protein (BRE:d2GFP) protein and mRNA, and phospho-Smad1/5/8 immunoreactivty in 13- to 17-somite embryos. A–F: Whole embryos were imaged for both d2GFP protein (A,C,E) and mRNA (B,D,F; green) and phospho-Smad1/5/8 immunoreactivity (magenta) (A2–F2). Note the enriched and overlapping d2GFP and phospho-Smad1/5/8 immunoreactivity in the tail and lower trunk regions. Higher magnification images of the tail bud region showed colocalization of d2GFP protein and mRNA and phospho-Smad1/5/8 immunoreactivity in some cells (arrowheads), although others expressed only d2GFP protein or mRNA, or phospho-Smad1/5/8. G–J: Expression of transcripts for known BMP-regulated targets id1, hey1, and msxE (H–J) were found in regions associated with the BRE promoter (G).

Acknowledgments

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