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Fig. 4 Zebrafish used in this study and melanophore pigmentation of control and F1 offspring from a cross between normal golden-type females and chimeric male fish. (A) Control offspring of a wild-type zebrafish at 2 days postfertilization (dpf). (B) Control offspring of a goldentype zebrafish at 2 dpf. (C) Control offspring of a cross between a golden-type female and wild-type male at 2 dpf. Phenotype is wild-type. (D) Offspring at 2 dpf of a cross between a golden-type female and a golden-type male chimera, in which a wild-type primordial germ cell (PGC) from an embryoid cultured for 2 days at 22°C was transplanted into a golden-type blastula. Phenotype is wild-type. (E) Offspring at 2 dpf of a cross between a golden-type female and a golden-type male chimera, in which a wild-type PGC from a cryopreserved/thawed embryoid cultured for 1 day at 22°C was transplanted into a golden-type blastula. Phenotype is wild-type. (F) Control offspring at 30 dpf of a cross between a golden-type female and a wild-type male. (G) Control offspring at 30 dpf of a cross between a golden-type female and a golden-type male. (H) Control offspring at 30 dpf of a cross between a wild-type male and a golden-type female. (I) Offspring at 30 dpf of a cross between a golden-type female and a goldentype male chimera transplanted with a wild-type PGC from a cryopreserved/thawed embryoid cultured for 1 day at 22°C. Bars in (A,B,C,D,E), 500 μm; Bars in (F,G,H,I), 2 mm.