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Fig. 1 Visualized green fluorescent protein (GFP)-positive cells obtained using the dissociated blastomere (DB) and cultured embryoid (CE) methods. (A) Isolation of blastodiscs at the late-blastula stage from embryos injected with GFP-nos1-3′UTR strand-capped mRNA. Large embryos were blastulae before operation, whereas small ones were isolated blastodiscs. (B) DBs cultured for 1 day in a dissociated condition in sodium citrate solution. (C) Embryoid from an isolated blastodisc cultured for 1 day in Ringer?s solution. Arrows indicate GFPpositive cells (primordial germ cells). Bars, 500 μm.
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Full text @ Int. J. Dev. Biol.