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Figure Caption
Fig. 2 Zebrafish primordial germ cells (PGCs) isolated from 10-15 somite stage embryos. (A) Zebrafish PGCs visualized by green fluorescence in a 15- somite stage embryo that had been de-yolked. (B) Fluorescence microscopic image of PGCs isolated under the enrichment mode. (C) vasa RNA expression (red fluorescence) in isolated PGCs counterstained with DAPI (blue fluorescence). (D) Fluorescence micrograph of a day 1 zebrafish embryo transplanted with a single, sorted PGC. Scale bars indicate 100 μm.
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Full text @ Int. J. Dev. Biol.