ZFIN Image: Felber et al., 2011, Fig. 5

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Figure Caption

Fig. 5 ptc1^-/- fish have enhanced osteoblastogenesis. osx staining at 96 hpf of wild type (A and C), ptc1^-/- (B) and ptc2^-/- fish (D) reveals that ptc1^-/- larvae have increased expression in the ceratohyal perichondrium and ptc2^-/- fish have no change. Expression at achondral sites of ossification is unaffected. Alizarin red staining at 168 hpf of wild type (E and G), ptc1^-/- (F) and ptc2^-/- fish (H) shows that some of the ptc1^-/- fish have increased perichondral ossification (7 out of 25 mutant fish) while ptc2^-/- fish are indistinguishable from their siblings. ptc1^-/- fish also have ectopic perichondral ossification (5/25) that bridges from the joint of the opercle to the hyomandibula (insets in E and F). Please see Fig. 2 for abbreviations and notes.

Acknowledgments

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Reprinted from Mechanisms of Development, 128(1-2), Felber, K., Croucher, P., and Roehl, H.H., Hedgehog signalling is required for perichondral osteoblast differentiation in zebrafish, 141-152, Copyright (2011) with permission from Elsevier. Full text @ Mech. Dev.