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Fig. 3

ID
ZDB-IMAGE-110317-45
Publication
Xie et al., 2011 - Zebrafish as a Model System to Study the Physiological Function of Telomeric Protein TPP1
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Figures for Xie et al., 2011
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Figure Caption

Fig. 3 zfTPP1 interacts with zfPOT1.

(A) Schematic representation of domain homology between human and zebrafish POT1. PBR, TPP1-binding region. (B) The RD domain is important for zfTPP1-zfPOT1 interaction. Extracts from 293T cells co-expressing GST-tagged zfPOT1 with FLAG-tagged full-length zfTPP1, zfTPP1-ΔRD (RD domain deletion mutant), or hTPP1 were immunoprecipitated with anti-FLAG antibodies, and analyzed by western blotting with the indicated antibodies. (C) The C-terminal region of POT1is important for its interaction with zfTPP1. Extracts from 293T cells co-expressing FLAG-tagged zfTPP1 or zfTPP1-ΔRD with GST-tagged full-length zfPOT1 or zfPOT1 C terminal domain (zfPOT1C) were immunoprecipitated with anti-GST antibodies and analyzed by western blotting with the indicated antibodies.

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